mouse embryonic fibroblasts mefs  (ATCC)

Journal: PLOS One

Article Title: In vitro culture of human-infecting Encephalitozoon spp. for genome sequencing with minimal host contaminant

doi: 10.1371/journal.pone.0344468

Figure Lengend Snippet: HFF-1 cells 100% confluent observed under phase-contrast inverted microscope with a 40X magnification and the Canon EOS REBEL T3i digital camera.

Article Snippet: The microsporidia cell culture was maintained until all 10 petri dishes reached 90–100% infection (approximately a month), following the herein described protocols. shows E. hellem ATCC 50451 infected HFF-1 cells (magenta arrows) after different times post-infection: at 7 days post-infection (dpi), we observed the formation of vacuoles in a few HFF-1 cells, as anticipated [ , ] (A); at 27 dpi most of the cells in the culture presented signs of infection by microsporidia (B); and at 32 dpi the spore-containing vacuoles showed a significant increase in size, occupying most of the host cell cytosol (C).

Techniques: Inverted Microscopy

Journal: PLOS One

Article Title: In vitro culture of human-infecting Encephalitozoon spp. for genome sequencing with minimal host contaminant

doi: 10.1371/journal.pone.0344468

Figure Lengend Snippet: HFF-1 cells infected with Encephalitozoon hellem ATCC 50451 at different post- infection days. E. hellem vacuoles in HFF-1 cell culture (magenta arrow) at 7 dpi (A), 27 dpi (B), and 32 dpi (harvest stage) (C).

Article Snippet: The microsporidia cell culture was maintained until all 10 petri dishes reached 90–100% infection (approximately a month), following the herein described protocols. shows E. hellem ATCC 50451 infected HFF-1 cells (magenta arrows) after different times post-infection: at 7 days post-infection (dpi), we observed the formation of vacuoles in a few HFF-1 cells, as anticipated [ , ] (A); at 27 dpi most of the cells in the culture presented signs of infection by microsporidia (B); and at 32 dpi the spore-containing vacuoles showed a significant increase in size, occupying most of the host cell cytosol (C).

Techniques: Infection, Cell Culture

Journal: Nature Communications

Article Title: HCMV infection disrupts barrier functions and promotes epithelial–mesenchymal transition in a cholangiocyte organoid model

doi: 10.1038/s41467-026-68962-8

Figure Lengend Snippet: a hiPSC-derived cholangiocyte-like cell (CLC) organoids were infected with HCMV TB40/E-eGFP at an MOI of 1. Representative images of mock- and HCMV-infected CLC organoids on day 10 PI are shown at 40× and 100× magnifications. b Number and c size of mock- and HCMV-infected CLC organoids on days 4 and 10 PI. For b , n = 132 and 102 for Day 4 PI Mock and HCMV; 208, 135 for Day 10 PI Mock and HCMV, respectively, measured from three biological replicates. For c , n = 6 biological replicates. Flow cytometry results showing percentage of GFP + cells in mock- and HCMV-infected CLC organoids on day 10 PI, shown as d dot plots or as a e column graph ( n = 6 biological replicates). f qPCR analysis of number of intracellular HCMV DNA copies per cell in mock-infected, HCMV-infected GFP - and GFP + cells, and Hs27 fibroblasts at 48 h PI. n = 9, 9, 6, and 4 biological replicates, respectively. g Representative images of in situ hybridisation of HCMV DNA probe (yellow), with GFP signals from HCMV-infected CLC organoid on day 10 PI or mock control, counterstained with DAPI (blue). Column graph shows quantification of percentage of probe⁺ and GFP⁺ cells ( n = 5 biological replicates). h Immunofluorescence staining of HCMV late protein pp28 (red) with GFP signals (green); i column graph shows percentage of pp28⁺, GFP⁺, and double-positive cells. j Supernatant (sup) from mock- and HCMV-infected CLC organoids were co-cultured with HFF-1 cells for 2 days and monitored for GFP signals ( n = 4 biological replicates). k RT-qPCR analysis of HCMV gene expression ( UL44, UL55, UL83, UL122, LAcmvIL10 , and LUNA ) in mock-infected, HCMV-GFP¯, and HCMV-GFP + cells from infected CLC organoids ( n = 6 biological replicates for UL44, UL55, UL83 , and UL122 ; n = 3 biological replicates for LAcmvIL10 and LUNA ). Scale bars with dimensions are shown. Data in graphs show the mean ± SD. Boxplots show centre line as median with box boundaries as 25 th to 75 th percentile, and whiskers as minima to maxima. One-way ANOVA was performed for ( b , c , f ). Student’s paired two-tailed t -test for ( e ). P values for b , P = 0.03 between Day 4 PI Mock vs HCMV, P < 0.001 between Day 10 PI Mock vs HCMV; for c , 0.62 between Day 4 PI Mock vs HCMV, P < 0.001 between Day 10 PI Mock vs HCMV; for e , P < 0.001 between Mock and HCMV; for ( f , P < 0.001 between GFP - and GFP + ; for k , for UL55 , UL83 , UL122 , UL138 , LAcmvIL10 , LUNA , between HCMV-GFP − and HCMV-GFP + , P = 0.01, 0.02, 0.01, <0.001 < 0.001, <0 . 001, respectively. * P < 0.05, ** P < 0.01, *** P < 0.001. Also see Figs. and .

Article Snippet: HFF-1 (#SCRC-1041) and Hs27 (#CRL-1634) cells are both human foreskin fibroblasts and were purchased from ATCC.

Techniques: Derivative Assay, Infection, Flow Cytometry, In Situ, Hybridization, Control, Immunofluorescence, Staining, Cell Culture, Quantitative RT-PCR, Gene Expression, Two Tailed Test